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شركــــــة
Bio Rad
الامريكــــــــة
متخصصة في
انتاج الاجهزة المختبرية و التحليلية مثل
Real Time PCR,
Gradient PCR, GEL Documentary,
Centrifuges, Elisa Systems and others
يمكن
الأطلاع على المزيد عند زيارة موقع الشركة
www.bio-rad.com
|
Bio Rad / USA
Specialized in manufacturing
Life Science products such as Real Time PCR,
Gradient PCR, GEL
Documentary,
Centrifuges, Elisa Systems and others.
Website:
www.bio-rad.com |
Download |
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PCR systems: First generation: Conventional PCR
Second generation: Real time PCR
Real Time PCR CFX96
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The CFX96™ optical reaction module converts the C1000 Touch™
thermal cycler chassis into the powerful and precise CFX96 Touch real-time PCR
detection system. This six-channel system combines advanced optical technology
with precise thermal control to deliver sensitive, reliable detection. Quickly
set up runs and monitor amplification traces in real time on the integrated LCD
touch screen. With up to five-target detection, unsurpassed thermal cycler
performance, unrivaled stand-alone functionality, and powerful yet easy-to-use
software, the CFX96 Touch system is designed to advance your qPCR.
Key Features and Benefits
With the CFX96 Touch real-time PCR detection system you can:
Set up your system quickly — easy installation and
factory-calibrated optics
Minimize sample and reagent use — up to 5-target multiplexing with
sample volumes as low as 10 µl
Optimize reactions in a single run — thermal gradient feature
Analyze data faster — visualize all run data at once and export
only the data you need in the format you want
Use advanced data analysis tools — normalized gene expression using
CFX Manager™ software
Configure the system to fit your needs — run without a computer,
run up to 4 systems from 1 computer, or integrate with the CFX automation
system for higher throughput
Streamline publication submission — include MIQE annotations and
generate RDML files using Biogazelle's qbasePLUS software
CFX Automation System for
Real-Time PCR
|
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The CFX automation system works with the
CFX Connect,
CFX96 Touch, and
CFX384 Touch real-time PCR detection systems
to enable walk-away, high-throughput operation in a simplified format that
does not
compromise
precision. The plug-and-go benchtop plate handler has the capacity to load up
to twenty 384-well PCR plates at a time.
This system
facilitates the automation of workflows, generation of large volumes of data,
and rapid data analysis. It is ideally suited to meet the high-throughput
requirements of today’s drug discovery workflows, letting you process up to
7,680 samples in a single run.
The CFX
automation system makes it easy to:
·
Maximize laboratory throughput by integrating one CFX96 or
CFX384 real-time PCR detection system with hands-free running of up to 20
plates
·
Improve experimental workflow and automation tasks to ensure
maximum productivity
·
Track samples using the integrated bar code
reader
·
Navigate, set up, and execute
multiple PCR experiments
using intuitive CFX automation control software
·
Define PCR protocols for an entire plate stack at once
·
Receive email notification with an attached data file or
report upon completion of a single run
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C1000
Touch thermal
cycler
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The C1000 Touch thermal cycler offers superior performance and a
large color touch screen for easy programming. This fully modular platform is able
to accommodate different throughput needs with easily interchangeable reaction
modules that swap in seconds without tools. Each PCR module has a fully
adjustable heated lid that supports a wide range of vessels and sealers,
including low-profile and standard-height PCR plates.
Features and Benefits
Quick and easy protocol programming — 2 programming options,
including the Protocol Autowriter, which automatically generates customized
standard, fast, and ultrafast protocols
Easy protocol editing — the large color touch screen and redesigned
navigation make editing and running protocols easy
Flexible platform — change throughput capability by linking up to 3
additional S1000™ thermal cyclers or adding PC control for up to 32 cyclers
Advanced file management — USB flash drive compatibility allows
universal protocol transfer and unlimited data storage
Thermal gradient — quickly and easily identify optimal annealing
temperatures by using the programmable temperature gradient.
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T100 _ thermal cycler PCR |
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The T100
thermal cycler is a small thermal cycler offering a comprehensive set of
convenient features in a small footprint. This compact thermal cycler features
an intuitive touch-screen user interface to make running PCR easy. Thermal
gradient technology allows you to quickly optimize your reaction in a single
run. With its robust design, the T100 system is a reliable personal thermal
cycler that delivers exceptional performance for years — test drive the T100
thermal cycler today.
Features and
Benefits of the T100 Thermal Cycler
Intuitive touch
screen — easy programming using a large color touch screen and intuitive
interface
Consistent
results — robust design ensures effective and consistent performance run to run
Easy
optimization — this gradient thermal cycler allows fast PCR optimization using
a unique thermal gradient
Easy protocol
management — protocols can be organized using personalized folders or a USB
flash drive
Small
space-saving footprint — the T100 is a compact thermal cycler that fits in any
laboratory
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Third
generation: Droplet digital PCR
QX-100
Droplet
digital PCR
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Bio-Rad
Laboratories presents Droplet Digital PCR (ddPCR) a novel combination of
limiting dilutions, end-point PCR, and Poisson statistics, yielding absolute
measurements of nucleic acid concentration. By generating uniformed
mono-dispersed droplets and using conventional probe based assays within a 96-
well plate environment, our system provides data orders of magnitude more
precise and sensitive than traditional qPCR. Bio-Rad's instrument, the QX100,
exceeds the ability of traditional qPCR techniques.A Breakthrough in
Quantitative PCR:
Droplet
Digital™ PCR
• Rare Event
Detection
Mutation
detection down to 0.001%
Single-cell PCR
detection
• Copy
Number Variations
Structural
genomics
Aneuploidy/Translocations
• Genotyping
• Absolute
Quantification
No standard
curve needed, no relative quantification
Single copy resolution
Unambiguous genotype calling
Applications
and Uses
·
Copy number variation
·
Rare sequence detection
·
Mutation detection
·
Gene expression analysis
·
miRNA analysis
·
Next generation sequencing sample
quantification
Specifications
for the QX100™ Droplet Digital™ PCR system
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Starting
sample size, μl
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20
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QX100 droplet
generator capacity, samples/cartridge
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8
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Droplets per
sample
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20,000
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QX100 droplet
reader throughput,
wells/hr
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32
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Sample
illumination
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Light-emitting
diodes
|
Sample
detection
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Photomultiplier
tubes
|
Detection
channels
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FAM, VIC
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Linear
dynamic range
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5 orders of
magnitude
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Precision
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+10%
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Droplets per
96-well plate, million
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1.4
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PX1™ PCR Plate Sealer |
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The PX1 PCR plate sealer provides
consistent and uniform sealing across an entire microplate. This semiautomated
heat sealer is ready to seal in less than 3 minutes. The PX1 sealer removes
human variability from plate sealing and minimizes sample evaporation from the
PCR workflow. This versatile instrument is compatible with a variety of
heat sealing films and foils and a wide range of PCR plates.
The PX1 PCR plate sealer features
an
intuitive touch-screen user interface. The thermal sealing process is
simplified by allowing sealing temperature and time to be modified with the
touch of a button.
Key Features and Benefits
·
Fast startup time — avoid delaying an experiment
while the sealer warms up
·
Extremely intuitive — save time programming the
instrument
·
Small footprint — accommodates crowded laboratory
benches
·
Quickly access sealing protocols — save time by easily using
stored protocols
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Heat up time, min
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<3
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Display, cm (in)
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10.9 (4.3) color touch screen
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Temperature range, °C
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100–190, off
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Time range, sec
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0.5–10.0
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Dimensions (W x D x H), cm (in)
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19.2 x 24.8 x 21.9 (7.6 x 9.8 x
8.6)
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Weight, kg (lb)
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7.3 (16)
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Power, watts
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840
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Voltage
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100–240 VAC, 50–60 Hz
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Comparative Specifications
for All Bio-Rad Real-Time PCR Detection Systems |
Feature
|
MiniOpticon™
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CFX
Connect™
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CFX96
Touch™
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CFX384
Touch™
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Excitation
range
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470–500
nm
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450–535
nm
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450–684
nm
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450–650
nm
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Light
source
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Light-emitting
diode (LED) array
(48 blue-green LEDs)
|
3
LEDs in optics shuttle
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6
LEDs in optics shuttle
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5
LEDs in optics shuttle
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Optical
detection
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2
photodiodes
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3
photodiodes
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6
photodiodes
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5
photodiodes
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Detection
range
|
523–700
nm
|
515–580
nm
|
515–730
nm
|
515–690
nm
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Multiplex
capability
|
Up
to 2 targets
|
Up
to 2 targets
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Up
to 5 targets
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Up
to 4 targets
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Gradient
range
|
35–99°C
|
30–100°C
|
30–100°C
|
30–100°C
|
Maximum
gradient span
|
16°C
|
24°C
|
24°C
|
24°C
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Maximum
ramp rate
|
2.5°C/sec
|
5°C/sec
|
5°C/sec
|
2.5°C/sec
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Dimensions
(W x D x H)
|
18
x 32 x 33 cm
|
33
x 46 x 36 cm
|
33
x 46 x 36 cm
|
33
x 46 x 36 cm
|
Weight
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7
kg
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21
kg
|
21
kg
|
21
kg
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Base
thermal cycler
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MJ
Mini™
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CFX
Connect™
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C1000
Touch™
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C1000
Touch™
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The
NGC family of chromatography system |
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NGC
family of chromatography system
The family of NGC medium-pressure
chromatography systems offers a single laboratory chromatography solution that
scales to fit your throughput requirements. Preconfigured systems are available
and are easily upgradable as your throughput and automation needs change.
The NGC system’s modular design
provides multiple configurations that each align with the requirements of
individual scientists. The system’s small footprint design allows the
instrument to be used on a lab bench, in a laboratory refrigerator, or in a
coldroom.
The preconfigured NGC Quest™ 10,
NGC™ Quest 10 Plus, NGC Scout™ 10, NGC™ Scout 10 Plus, and NGC Discover™ 10
systems are designed with increasing automation and throughput to serve a wide
range of laboratory needs. Additionally, each system is also completely open to
individual customization through the selection of applicable modules suitable
to the application at hand. To learn more, watch the NGC system tour.
Prepacked Analytical-Scale Chromatography Media Cartridges by
Application
Common Chromatography
Method Elements
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Flow
cytometer: S3 Cell sorter |
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The S3 cell sorter is the first
truly walk-up automated cell sorter available to scientists. Expertly
engineered with revolutionary precision and automation, the S3 cell sorter is a
benchtop cell sorter equipped with one or two lasers and up to four fluorescent
detectors plus scatter detectors utilizing traditional jet-in-air technology.
Samples can be sorted at high speeds while maintaining sensitivity and high
purity. Cell sorting has never been easier for the most common sorting
experiments. Small in footprint, the S3 cell sorter brings affordable,
dependable, and simplified cell sorting to every researcher.
Key Features and Benefits
Walk-up cell sorting — provides
automation to simplify setup and optimization
Automated setup — creates precision
sorting without user input or constant monitoring; fully automated drop delay
calculation, stream-to-laser alignment, droplet break-off monitoring, and
feedback ensure sort accuracy
Benchtop footprint — includes
space-saving, internalized fluidics and temperature control system to fit in
any lab
High speed and high purity — sorts
cells faster while maintaining high sensitivity and yield
2-way cell sorting — sorts 2
different defined populations at the same time
Automatic cleaning — reduces
carryover with a dual-position loading stage that ensures the sample line is
clean between sort runs
Specifications
For Flow
cytometer: S3 Cell sorter
Acquisition rate, events/sec
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Up to 100,000
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Sorting rate, events/sec
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Up to 30,000
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Sorting purity
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>99% pure
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Nozzle size, µm
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100
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Sorting direction
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2-way sorting
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Sorting collection
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Up to 5 x 5 ml sample tubes each
direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
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Lasers
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Primary: 488 nm 100 mW
Optional: 561 nm 100 mW
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Detection
|
Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
Up to 4 fluorescence detectors with PMT
Minimum resolution: 0.5 µm
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Sensitivity, MESF
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<125 for FITC and PE
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Temperature range, °C
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Sample and collection temperature
control system: 4–37
Peltier solid state system
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Fluidics
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Onboard fluidics and dilution of
8x sheath fluid with deionized water
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Data format
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FCS 3.1
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Dimensions (W x D x H), ft/cm
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2.3 x 2.1 x 2.1 / 70 x 65 x 65
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xMark™ Micro plate Absorbance
Spectrophotometer ( Elisa Systems ) |
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With its unique imaging
capabilities, monochromator design, and spectral scanning feature (which
eliminates the need for filters), the xMark™ microplate absorbance
spectrophotometer can find the best wavelength within a broad range for any
photometric application. The xMark spectrophotometer is also flexible enough to
read all standard microplates, from 6- to 1,536-well formats. Using Microplate
Manager® software, the xMark spectrophotometer can perform a wide
range of end-point and kinetic applications with spectral scanning within low
UV to infrared wavelengths. Extensive data analysis includes comprehensive
curve-fit models and automated data export in single-plate or batch formats.
Benefits include:
·
Monochromator-based system for
maximum optical efficiency and flexibility in photometry reading
·
Spectral scanning to define the
best absorbance wavelength for assays
·
Imaging capabilities for pixel
intensity data in a single well or entire plate
·
Ability to scan custom plates
·
Flexible control and data
analysis with PC or Mac software
·
Incubator with programmable
temperature control
The
ImmunoWash™ 1575 microplate washer is essential for performing accurate and
reproducible immunoassays in a microplate format, such as ELISA.
Features
and Benefits
·
Choose from a variety of
preprogrammed wash sequences
·
Design, store, and reproducibly
perform special wash routines
·
Maximize wash efficiency by
adapting to various liquids and buffers, assay components and conditions
·
Exercise complete control of
needle position (horizontal and vertical) and aspiration/dispensing rates
Applications
and Uses
·
Suitable for autoclave
sterilization
·
Compatible with 96-well
microplates that have flat-, U-, or V-bottom wells and strips
·
Registered with the FDA as a
medical device for various clinical chemistry applications
Specifications for Micro plate Absorbance
Spectrophotometer (Elisa Systems)
Optical
|
|
Wavelength range
|
200–1,000 nm
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Photometric methods
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Single and dual wavelength
|
Monochromator step size
|
1 nm
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Bandwidth
|
5 nm
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Wavelength accuracy, minimum
|
±2.0 nm
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Wavelength repeatability
|
±0.2 nm
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Imaging resolution
|
0.5, 0.75, 1.0 mm
|
Performance
|
|
Indication range
|
0–4.0 OD
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Resolution
|
0.001 OD
|
Linearity
|
≤2.0%, 0–3.0 OD at 405 nm
|
Accuracy
|
±1.0% or 0.015, 0–3.0 OD
|
Reproducibility
|
96-well plate; ≤1.0% or 0.005, 0–3.0 OD at 405 nm
|
Stability and drift
|
≤0.010 at OD = 1 at 490 nm
|
Read time
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8 sec/96-well/single wavelength; 15 sec/384-well/single
wavelength; 35 sec/1,536-well/single wavelength
|
Physical
|
|
Compatible plates
|
6- to 1,536-well and custom microplates
|
Maximum plate height
|
21 mm
|
Immunowash
Microplate Washer
Electrical
|
|
Voltage
|
200/100 VACm 50/60 Hz
|
Consumption
|
100 VA maximum
|
Physical
|
|
Dimensions (W x D x H), cm (in)
|
32.5 x 40.1 (plate carrier
in)/52.6 (plate carrier out) x 19.3 (12.8 x 15.8/20.7 x 7.6)
|
Weight, kg (lb)
|
9.6 (21)
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Operating conditions
|
15–30°C, 15–85% relative humidity
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Hardware
|
|
Manifolds
|
8- and 12-way
|
Vacuum
|
Integrated vacuum pump, 9 L/min
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Waste bottle volume, ml
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2000
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User interface
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2 x 20 character LCD screen; 5
diaphragm keys flat keyboard
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ChemiDoc™
XRS+ System & ChemiDoc
MP System
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|
The ChemiDoc MP system is a full-feature instrument for gel or
western blot imaging. It is designed to address multiplex fluorescent western
blotting, chemiluminescence detection, and general gel documentation
applications.
Its features are based on CCD high-resolution, high-sensitivity
detection technology, and modular options to accommodate a wide range of
samples and support multiple detection
methods. The system is controlled by Image Lab™ software to
optimize performance for fast, integrated, and automated image capture and
analysis of various samples.
Features and Benefits
Multiple imaging capabilities
— the ChemiDoc
MP imager can accommodate a variety of sample types and detection methods including
multiplex fluorescent western blotting. It is the perfect imager to accompany
your protein and DNA electrophoresis runs as well as your western blotting
experiments. It delivers quantitative, reproducible results for fluorescence,
chemiluminescence, and colorimetric detection
Stain-free technology
— UV-induced
fluorescence labeling of proteins in the stain-free gels allows a 2 hr
Coomassie gel-staining protocol to be condensed into a 5 min stain-and-image
step. Stain-free gels are western blot compatible — using the V3 Western
Workflow™, check your electrophoresis results and blot transfer quality prior
to western blotting
High-sensitivity blot detection
— the ChemiDoc
MP imaging system offers advanced detection technology that determines optimal
exposure, even for faint or intense samples. Superior sensitivity is achieved
for chemiluminescence and multiplex fluorescence detection and for colorimetric
gel and blot documentation
Superior Image Quality — Exceptional
dynamic range enables visualization of faint and intense bands on same blot or
gel. Images are always in focus at any zoom level to ensure publication-ready
images in seconds.
Ease of Use — precalibrated system provides the
precise focus for any zoom setting or sample height; automated hands-free
operation ensures consistent, reproducible, and high-throughput performance
Specifications
Applications
|
|
Chemiluminescence
|
Yes
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Fluorescence
|
Yes
|
Colorimetry/densitometry
|
Yes
|
Gel documentation
|
Yes
|
Hardware Specifications
|
|
Maximum sample size
|
28 x 36 cm
|
Maximum image area
|
26 x 35 cm
|
Maximum image area
|
(For standard UV-excited gels) 25
x 26 cm
|
Excitation source
|
Epi-white light and trans-UV (302
nm) are standard (optional 365 nm lamp available); optional trans-white
conversion screen, XcitaBlue™ conversion screen, and epi-red, epi-green, and
epi-blue LED modules available
|
Illumination control
|
8 modes available. Trans-UV,
epi-white, and no illumination for chemiluminescence are standard; epi-red,
epi-green, epi-blue, trans-white, and XcitaBlue conversion screens are
optional
|
Detector
|
Supercooled CCD
|
Image resolution
|
4 megapixels
|
Pixel size (H x V)
|
6.45 x 6.45 µm
|
Cooling system
|
Peltier
|
Camera cooling temperature
|
-30°C absolute and regulated
|
Filter holder
|
6 positions (5 for filters, 1
without filter for chemiluminescence)
|
Emission filters
|
1 included (standard), 4 optional
|
Dynamic range
|
>4.0 orders of magnitude
|
Pixel density (gray levels)
|
65,535
|
Dynamic flat fielding
|
Application specific; for all
applications
|
Instrument size (L x W x H)
|
36 x 60 x 96 cm
|
Instrument weight
|
32 kg
|
Operating Ranges
|
|
Operating voltage
|
110/115/230 VAC nominal
|
Operating temperature
|
10–28°C (21°C recommended)
|
Operating humidity, noncondensing
|
<70%
|
Automation Capabilities
|
|
Workflow automated selection
|
Application driven; user selected
or recalled by a protocol
|
Workflow automated execution
|
Controlled by a protocol via
application-specific setup for image area, illumination source, filter,
analysis, and reporting
|
Workflow reproducibility
|
100% repeatability via recallable
protocols; from image capture to quantitative analysis and reports
|
Autofocus (patent pending)
|
Precalibrated focus for any zoom
setting or sample height
|
Image flat fielding**
|
Dynamic; precalibrated and
optimizor every application
|
Autoexposure
|
2 user-defined modes (intense or
faint bands)
|
Gel
Doc EZ System
|
|
The Gel Doc EZ system is a compact and automated gel imaging
instrument designed to yield publication-quality images and analyzed results with
just the push of a button. The Gel Doc EZ system provides unparalleled
flexibility with the use of four application-specific trays:
UV tray for ethidium bromide staining of DNA gels and fluorescence
imaging
White tray for Coomassie, copper, silver, and zinc stains
Blue tray for nucleic acid applications with SYBR®
stains
Stain-free tray for stain-free imaging with
Bio-Rad
stain-free gels
The intuitive
Image
Lab™ software bundled with the Gel Doc EZ gel imaging system includes
capabilities such as auto image capture, auto analysis, user preferences, and
many other features, making gel imaging and analysis incredibly easy.
Features and Benefits of the Gel Doc EZ system
Easy to use — Researchers unfamiliar with the Gel Doc
EZ imaging system can learn to use it quickly and easily because there is no
need for manual control of filters, lens, or lights. Create a default protocol
once, then use the green button on the front of the instrument to use these gel
imaging and analysis settings time and again.
Publication-quality images and full data analysis —
Get clean and smooth publication-ready images with decreased pixelation when
images are cropped or zoomed. Output analyzed results including relative
molecular weights, bands quantitation, Excel reports, PDFs, and more in a
matter of minutes.
Time and space savings — Obtain results faster
without spending time on various steps in image acquisition and save valuable
bench space using this compact system.
Modular design and flexible options — Use specific
trays for different gel imaging applications. Clearly defined and color-coded
trays eliminate confusion. Purchase only what you need and upgrade as your
research needs change.
Stain-free technology — Condense your 2 hr Coomassie
protocol into a 5 min stain-and-image step with this stain-free gel imaging
system.
Bio-Rad
stain-free gels are western blot compatible, allowing you to check
electrophoresis results and quality prior to western blotting. These “green”
gels provide equal or better sensitivity than Coomassie staining and eliminate
organic waste disposal problems.
|
|
Electrophoresis:
Experion
Automated Electrophoresis System
|
|
The Experion automated electrophoresis station performs all of the
steps of gel-based electrophoresis in one compact, durable unit. It automates
analysis by combining electrophoresis, staining, destaining, band detection,
and imaging into a single, 30 min step. It's an electrophoresis cell, power
supply, and imager built into a single device and is designed for easy, precise
operation:
Highly accurate laser provides precise fluorescence detection
USB port allows easy installation and maximum usability
Built-in power supply reduces cost and saves bench space
The following types of kits are available for analysis of proteins,
RNA, and DNA:
Experion starter kits
Experion protein analysis kits
Experion RNA analysis kits
Experion DNA analysis kits
The Experion automated electrophoresis system can be used upstream
and downstream of a number of other nucleic acid- and protein-application
focused products from Bio-Rad.
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OMA International Group
|
For more information, please
visit our website (www.omatrade.com).
Contact Information:
Mobile: 00 964 77 0 444 6566
Email:
sales@omatrade.com ,
support@omatrade.com,
zaid@omatrade.com |
Al-Shareif Building, Street 9, District 102, Alweyah
Baghdad-Iraq - P.O BOX: 3056
|
www.omatrade.com
www.facebook.com/omainternational |
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